|
KMID : 0363819800140010045
|
|
Korean Journal of Nuclear Medicine
1980 Volume.14 No. 1 p.45 ~ p.56
|
|
|
Studies on the Preparation of T₃-BSA, T₄-BSA Conjugates, and Radioimmunoassay
Use of the Produced Antisera
|
|
|
|
|
Abstract
|
|
|
T3-BSA and T,-BSA conjugates were prepared and identified spectrophotometrically. The Amax of the conjugates was just coincided with that of BSA, but the molar extinction coefficients of the conjugates were generally larger than that of BSA itself. The molar
ratios of T3: BSA and T4: BSA in the prepared conjugates were found to be 9 : I and 5 : 1, respectively. The titers of the T3 antisera were generally higher (max. 1.5 x 10 : 1) than those of T, (max.2x 103 : 1), and the average cross reactivity of the T3 antibody with T, was lower (0.45%) than that of T, antibody with T3(3-47o).
The results of.the study indicate that the predominant cause of the lower titers and the lower specificity of the T, antisera comparing with those of T3 is mainly due to the unstability of the T,-BSA and consequent degradation of the conjugate to T3 BSA during preparation, purification, and even during immunization. The lower molar ratio of T4 to BSA in the preparation stage is also considered to be a minor factor.
By measuring T3, T, levels in the reference control serum, it has been confirmed that the prepared antisera can sufficiently be utilized, respectively, in the established radioimmunoassay systems.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|